Figure 2.

rtel-1 Is Synthetic Lethal in Combination with pole-4 Deletion

(A) Progeny viability in N2(wild-type), pole-4, and rtel-1 single mutants and rtel-1; pole-4 double mutants (^∗∗∗∗p < 0.0001; n.s., not significant).

(B) Top: representative images of Cy3-dUTP incorporation in mitotic nuclei in N2(wild-type), pole-4, and rtel-1 single mutants and rtel-1; pole-4 double mutant worms. Bottom: bar graphs showing the percentage of Cy3-dUTP-positive cells in the described genetic backgrounds (^∗∗∗∗p < 0.0001; n.s., not significant).

(C) Average number of mitotic nuclei counted in a single plane of focus within 45 μm of the distal tip cell as a measure of mitotic arrest and checkpoint activity in the genotypes shown. N2(wild-type) animals treated for 24 h with 40 mM hydroxyurea (HU) were used as a control (^∗∗∗∗p < 0.0001).

(D) RPA staining of mitotic zones in the indicated genotypes (see also Figure S2B for quantification).

Images are composites of several images stitched together. Error bars in all graphs represent standard deviation (SD) of the mean.