Figure 7.

Impaired Fork Progression and Increased Fork Stalling in RTEL1-POLE4 Double Knockout Cells

(A) Representative DNA fiber immunofluorescence from RTEL1^F/FPOLE4^+/+ and RTEL1^F/FPOLE4^−/− MEFs infected with GFP-CRE or empty GFP.

(B) Bar graphs showing replication fork speed (measured as IdU track length/min) from RTEL1^F/FPOLE4^+/+ and RTEL1^F/FPOLE4^−/− MEFs transduced or not with CRE. ^∗∗∗∗p < 0.0001.

(C). Analysis of replication fork symmetry from RTEL1^F/FPOLE4^+/+ and RTEL1^F/FPOLE4^−/− MEFs infected with GFP-CRE or empty GFP. Total number of newly established replication forks analyzed is indicated.

(D) Bar graphs showing inter-origin distance measurements from RTEL1^F/FPOLE4^+/+ and RTEL1^F/FPOLE4^−/− MEFs transduced or not with CRE recombinase (^∗∗p < 0.01, ^∗∗∗p < 0.001). Error bars represent standard deviation (SD) of the mean. Scale bars, 10 μm.