REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat Anti-Rat IgG (H+L) Antibody, Alexa Fluor 594 Conjugated	Thermo Fisher	Cat#A-11007; RRID: AB_141374
Rabbit Anti-Mouse IgG (H+L) Antibody, Alexa Fluor488 Conjugated	Thermo Fisher	Cat#A-11059; RRID: AB_142495
Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor488 Conjugated	Thermo Fisher	Cat#A-11034
Rabbit polyclonal anti-53BP1	Novus Biologicals	Cat#NB100-304; RRID: AB_10003037
Mouse monoclonal γH2AX clone JBW301	Millipore	Cat#05-63; RRID: AB_309864
Rat monoclonal anti-BrdU	AbD Serotec	Cat#OBT0030
Mouse monoclonal anti-BrdU	Becton Dickinson	Cat#347580
Chemicals, Peptides, and Recombinant Proteins
Adenovirus Ad-Cre-GFP	Vector Biolabs	Cat#1700
Adenovirus Ad-GFP	Vector Biolabs	Cat#1060
CldU	Sigma-Aldrich	Cat#C6891
IdU	Sigma-Aldrich	Cat#I7125
EdU	Thermo Fisher Scientific	Cat#A10044
Benzonase	Novagen	Cat#71206-3
DAPI	SIGMA	Cat#10236276001
Critical Commercial Assays
FiberPrep® (DNA Extraction Kit)	Genomic Vision	Cat#EXTR-001
Lipofectamine 2000	Thermo Fisher	Cat# 11668027
QIAprep Spin Miniprep Kit	QIAGEN	Cat#27106
Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit	Thermo Fisher	Cat#C10425
Experimental Models: Mouse Strains
Pole4tm1(KOMP)Vlcg	Bellelli et al., 2018	N/A
RTEL1 f/f	Vannier et al., 2012	N/A
Experimental Models: Cell Lines
Mouse Embryonic Fibroblasts RTEL1f/fPole4-+/+	This study	N/A
Mouse Embryonic Fibroblasts RTEL1f/fPole4−/−	This study	N/A
Human HEK293 cells	The Francis Crick Institute Cell Services	N/A
Experimental Models: C. elegans strains
C. elegans: WT, Bristol (N2) background	CGC	N2
FX1866 rtel-1(tm1866)	Barber et al., 2008	DW663
FX4613 Y53F4B.3 pole-4(tm4613)	CGC	N/A
FX1937 mus-81(tm1937)	Barber et al., 2008	N/A
DW238 rtel-1(tm1866) mus-81(tm1937)/hT2[bli-4(e937) let-?(q782) qIs48](I;III)	This study	N/A
VC13 dog-1(gk10)	Cheung et al., 2002	N/A
VC193 him-6(ok412),	Wicky et al., 2004	N/A
RB1279 rfs-1(ok1372)	Ward et al., 2007	N/A
CB1487 him-9(e1487)	Saito et al., 2009	N/A
Software and Algorithms
Adobe Photoshop CC	Adobe	https://www.adobe.com/es/products/photoshop.html
ImageJ	NIH	https://imagej.nih.gov/ij/
Volocity 6.3	PerkinElmer	http://www.perkinelmer.com/lab-products-and-services/resources/cellular-imaging-software-downloads.html
GraphPad Prism 7	GraphPad	https://www.graphpad.com/