Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul;1867(7):118709. doi: 10.1016/j.bbamcr.2020.118709

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 2 — Immunoblot analysis of fibroblast homogenates from MFF-deficient patients. Antibodies were directed against peroxisomal 3-ketoacyl-CoA thiolase (upper panel) or peroxisomal acyl-CoA oxidase 1 (ACOX1; lower panel). Lanes 1–3, MFF-deficient patient fibroblasts MFF^Q64⁎ [7], MFF^{L62Pfs⁎13+R298⁎} [6] and MFF^E153Afs⁎5 [6], respectively. Lane 4: control subject, Lane 5: fibroblasts of a patient with Zellweger Spectrum Disorder (ZSD). Results show normal proteolytic processing of 3-ketoacyl-CoA thiolase (40-kDa) and ACOX1 (50- and 20-kDa) in the MFF-deficient cell lines, whereas in the ZSD line the unprocessed bands of 3-ketoacyl-CoA thiolase (44-kDa) and ACOX1 (70-kDa) are present. Note that the protein band above the 70 kDa band of ACOX1 is non-specific.