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. 2020 Jul;1867(7):118709. doi: 10.1016/j.bbamcr.2020.118709

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© 2020 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Suppl. Fig. S1 — Re-introduction of MFF in MFF-deficient patient fibroblasts. MFF-deficient patient fibroblasts [mutation Q64* [7]] were transfected with Myc-MFF using microporation. Cells were processed for immunofluorescence microscopy 2–3 h after transfection using antibodies directed to the Myc-tag and PEX14, a peroxisomal membrane marker. Note the localisation of MFF in spots on elongated peroxisomes (upper panel; arrowheads), the appearance of shorter peroxisomes due to peroxisome division (middle panel), and the restoration of the normal, spherical peroxisome morphology (lower panel). Higher magnification of boxed regions is shown. Scale bars, 10 μm; magnification, 5 μm.