Suppl. Fig. S2.

The peroxisomal body is import-competent. MFF-deficient fibroblasts were transfected with GFP-SKL and grown on 3.5-cm glass bottom dishes. Photo-bleaching experiments were performed after 24–48 h using a Visitron 2D FRAP system. The entire organelle (peroxisome body and short tubule) was photo-bleached (0 min) and recovery of GFP-SKL fluorescence monitored over a period of 10 min (A). Note that GFP-SKL fluorescence was observed in the peroxisome body (arrowheads), but not in the peroxisome tubule, indicating that recovery is due to import of GFP-SKL into the peroxisome body. (B) Quantification of fluorescence intensity. Data are presented at the mean grey value for each increment along the length of the peroxisome. Scale bar, 5 μm.