Suppl. Fig. S3.
HsPEX3(1–44)-EGFP is targeted to mitochondria when peroxisomes are lost. Human control (C109) or MFF-deficient (MFFQ64⁎) fibroblasts were transfected with a plasmid coding for HsPEX3(1–44)-EGFP to induce peroxisome degradation and processed for immunofluorescence after 24 and 48 h using antibody against mitochondrial ATP synthase (ATPB). Note the mistargeting of HsPEX3(1–44)-EGFP to mitochondria (arrowheads). Furthermore, mitochondrial morphology is altered including fragmentation and clustering. Scale bars, 10 μm, magnification, 2 μm.