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Primers sequences for GAPDH, IL-1β, IL-6, and TNFα mRNA detection.
| Gene | Amplicon size (bp) | Sequence (5′ to 3′) | Reference |
|---|---|---|---|
| GAPDH | 96 | F: AATTCCACGGCACAGTCAAGG | [10] |
| R: CATTTGATGTTGGCGGGATC | |||
| IL-1β | 178 | F: CTGGTGCTGTCTGGCTCATA | [10] |
| R: TTCCCGTCTTTCATCACACA | |||
| IL-6 | 333 | F: TGCCTGACAAGAATCACTACT | [44] |
| R: GAACTACAGCAATCTTAGATG | |||
| TNFα | 250 | F: TGGCCTGCAACTAATCAACC | [10] |
| R: GTGTGGAAGGACATCCTTGG |
F: forward; R: reverse.
