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. 2020 May 26;8(10):1848–1858. doi: 10.12998/wjcc.v8.i10.1848

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©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Cross electro-nape acupuncture inhibited cerebral hemorrhage -mediated necrosome formation in vivo. A and B: Propidium iodide staining (A) and propidium iodide positive rate (B) in the brain tissues of experimental rats; C: Protein level of RIPK1, RIPK3, p-RIPK3, MLKL and p-MLKL in the brain tissues of experimental rats; D-H: Quantitative analysis of RIPK1 (D), RIPK3 (E), p-RIPK3 (F), MLKL (G) and p-MLKL (H); I-K: RIPK1 and RIPK3 interaction in the brain tissues of experimental rats using IP assay (J) and quantitative analysis (K) of the IP assay. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. CENA: Cross electro-nape acupuncture; CH: cerebral hemorrhage; RIPK: Receptor interacting protein kinase; MLKL: mixed lineage kinase domain like pseudokinase.