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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Mol Cancer Res. 2019 Oct 18;18(1):105–117. doi: 10.1158/1541-7786.MCR-19-0239

Figure 4.

Figure 4.

IDO1 expression alone is not the only factor in determining its activity. (A) Diagram illustrated that tryptophan can be used to generate serotonin and KYN. (B) Relative mRNA levels of IDO1. Total RNAs extracted from these cells were reverse-transcribed and subsequently used as template for real-time quantitative PCR. GAPDH was used as internal control. The results shown in the graph were calculated with the ΔΔCt method by setting the IDO1 mRNA level of LL24 cells as 1 (*p=0.005, **p=0.01). (C) Immunoblot of lung cancer cell lines treated with and without IFNγ (20ng/ml) for 24h. No significant differences in IDO1 protein expression levels were observed between parental and CR cells. (D) Significantly higher IDO1 activity was found in CR cells when compared to parental cell counterparts (*p=0.02, **p=0.03) and was further enhanced with IFNγ (20ng/ml) treatment for 24h (***p=0.03, ****p=0.008).