Figure 6.

In vivo changes in immune cells in the spleen of normal mice injected intravenously with 1 mg/kg of GBex. Mice received intravenous injections of GBex in PBS (1 mg/kg or equivalent volume) every 3 days for 16 days (see the schedule in Supplementary Figure 1). Animals were sacrificed and spleens were harvested, dissociated to obtain a single-cell suspension. The frequency of immune cells was determined by immunostaining and flow cytometry. The percentages of NK (CD45⁺ NKp16⁺), CD8⁺ T cells (CD45⁺CD3⁺CD8⁺), CD4⁺ T cells (CD45⁺CD3⁺CD4⁺), T-regulatory cells (CD4⁺FOXP3⁺), macrophages (CD45⁺CD11b⁺F4/80⁺), M1-like macrophages (CD45⁺CD11b⁺CD80⁺CD86⁺), M2-like macrophages (CD45⁺CD11b⁺CD206⁺), and MDSCs (CD45⁺CD11b⁺Gr1⁺) were determined in the spleen. The values represent the mean values ± SEM from 7 animals. *Indicates a significant difference from the CTRL (P < .05) as determined by Student’s t-test.