Table 1.

Primary antibodies and dilutions for immunoblotting.

Antibodies	Manufacturer	Dilution	Identified Band size (s)
MMP-1 (M)	R&D Systems	1:1,000	52 & 57 kDa
MMP-2 (M)	R&D Systems	1:1,000	72 kDa
MMP-3 (P)	Chemicon	1:1,000	57 & 59 kDa
MMP-9 (P)	Chemicon	1:1,000	92 kDa
MMP-24 (P)	Chemicon	1:1,000	64 kDa
TIMP-1 (P)	Chemicon	1:20,000	28 kDa
TIMP-2 (P)	Chemicon	1:2,000	21 kDa
TIMP-3 (M)	Calbiochem	1:200	27 kDa
TIMP-4 (P)	R&D Systems	1:7,000	29 kDa
GAPDH (P)	R&D Systems	1:20–40K	36 kDa

^*P: polyclonal rabbit anti-human antibody; M: monoclonal mouse anti-human antibody.