Log2 abundances normalized to HC in ELL (red) and EDL (blue) patients for early Lyme disease vs HC biosignature MFs classified as eicosanoids (A), bile acids (B), lyso-PAF and lysoPCs (C), acylcarnitines (D), sphingolipids (E), and 5-oxo-L-proline (F) for replicate 1, left and replicate 2, right. Metabolites that were present on the ELL vs EDL biosignature list are noted with #. Data is presented as the mean +/− 95% confidence interval. Dotted line at zero indicates HC baseline. Significance between the patient cohort and HCs is indicated by: *, p < 0.05; **, p < 0.01; ***, p < 0.001, ****, p < 0.0001. Panel B significance (p < 0.05) between ELL and EDL is indicated by * over a solid line between the groups.