Fig. 2.

RNA interference targeting MAST1 impairs the neuronal differentiation of SH‐SY5Y cells. SH‐SY5Y cells were infected with sh‐MAST1 or control (sh‐neg) lentiviruses. (A) Western blot analysis of MAST1 protein expressions in noninfected, control (sh‐neg), and sh‐MAST1 lentiviruses infected SH‐SY5Y cells. (B) Western blot analysis of MAP2 protein expression in control (sh‐Neg), or sh‐MAST1 lentiviruses infected SH‐SY5Y cells, which were cultured in the absence or presence of RA for 8 days. (C) Immunostaining of neuronally differentiated SH‐SY5Y cells, which were cultured in the presence of RA for 8 days, with anti‐MAP2 antibody. The neurite length was calculated in differentiated SH‐SY5Y cells (arrows: representative neurite). Scale bars, 200 μm. Data are depicted as means ± SD of at least three independent experiments. ***P < 0.001, as determined by the two‐tailed unpaired Student's t‐test.