Fig. 3.

MAST1 knockdown affects the cell cycle exit in SH‐SY5Y cells. SH‐SY5Y cells were infected with a control (sh‐neg) or sh‐MAST1 lentiviruses. After 4 days of culture in the absence or presence of RA, phases of the cell cycle were detected by flow cytometry. (A) Flow cytometry data showed distribution of the cell cycle. Percentages of cells in each phase of the cell cycle are depicted in the graph on the right. (B) Representative photomicrograph of EdU immunostaining. SH‐SY5Y cells that were cultured in the presence of 10 μm RA for 4 days and incubated with EdU for 2 h before fixation. The percentage of EdU‐positive cells was determined. Scale bars, 100 μm. Data are depicted as means ± SD of at least three independent experiments. **P < 0.01, ***P < 0.001 versus undifferentiated, as determined by one‐way ANOVA followed by the LSD post hoc test.