Fig. 2.

The influence of GHR on the growth of breast cancer cells. The expression of GHR knockdown was detected by western blotting. The results showed that GHR expression was significantly decreased in MDA‐MB‐231 cells (A) and MCF‐7 cells (B) with siGHR transfection. (C, D) Control and GHR knockdown MDA‐MB‐231(C) and MCF‐7 cells (D) were analyzed by colony formation. (E, F) Control and GHR knockdown MDA‐MB‐231 (E) and MCF‐7 cells (F) were examined for cell proliferation ability by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tetrazolium bromide assay. Data represent means ± SEM; ***P < 0.001, as determined by Student’s t‐test. All experiments were repeated three times (n = 3).