Fig. 2.

Different requirements of SSBP1 for the activation of mitochondrial chaperone genes. (A) Knockdown of HSF1 or SSBP1. MEF cells were infected with an adenovirus expressing scrambled RNA (SCR) or shRNA for SSBP1 (SSBP1‐KD) or HSF1 (HSF1‐KD) for 2 h, maintained with normal medium for 70 h. Cell extracts were prepared and subjected to western blotting. (B–D) Activation of the mitochondrial chaperone genes in SSBP1‐ or HSF1‐knockdown cells. SSBP1 or HSF1 was knocked down as described in (A). The cells were treated with 10 μm GTPP (B), 5 μm CDDO (C), and 20 μm rotenone (D) for 6 h. mRNA levels of HSP60, HSP10, mtHSP70, and HSP70 were quantified by RT‐qPCR (n = 3). Mean ± SD is shown. Asterisks indicate P < 0.01 or 0.05 by Student's t‐test (ns, not significant).