Skip to main content
. 2020 Apr 27;11(6):1594–1602. doi: 10.1111/1759-7714.13435

Figure 2.

Figure 2

Effect of overexpression of Cx43 and AKAP95 on their combination with cyclin E1/E2. (a) Total cell protein was extracted from A549 cells. Mouse anti‐Cx43 antibody was used for Co‐IP. Co‐IP products were detected using western blot analysis with cyclin D1 (rabbit), cyclin D2 (mouse), cyclin D3 (mouse), cyclin E1 (rabbit), and cyclin E2 (rabbit). (b) Total protein of 500 μg extracted from A549 cells was taken. Mouse anti‐AKAP95 antibody 4 μg and mouse anti‐Cx43 antibody 4 μg were used for Co‐IP. An equivalent buffer for Co‐IP product resuspension was taken. Rabbit antibodies AKAP95, cyclin E1, and cyclin E2 were applied to detect the Co‐IP product of AKAP95 antibody (row 1–3). Rabbit antibodies Cx43, AKAP95, cyclin E1, and cyclin E2 were used to detect the Co‐IP product of Cx43 antibody by western blot analysis (row 4–7). The ratio of Cx43+ group and AKAP95+ group to control group was taken as a statistical variable for comparison. The single‐sample t‐test was used to analyze the comparison of gray values between the experimental and control groups. A single‐factor ANOVA method was used to compare the gray values among different groups. *P < 0.05.