A, BALB/c mice were inoculated s.c. on day 0 with 1×105 CT26 colon carcinoma cells. On day 14 when the average tumor diameters were 6.4 – 6.7 mm, the mice were started on twice weekly i.t. injections of 20μg IgG, 200 μg CpG/mouse, or 200 μg CpG + 20μg CD80-Fc/mouse. I.t. injections were continued for three weeks. Graphs show average tumor volumes ± SD, percent surviving mice, and tumor growth in individual mice. Day 0 is the day of tumor inoculation. n= 5 mice/group. Statistical analysis was performed using pairwise comparison test.