Figure 5. CD80-Fc prevents CTLA4-mediated suppression by saturating CTLA4.

PHA-activated human PBMC, PDL1+ C8161 cells, and CD80-Fc (2 μg/ml) were incubated with increasing amounts of soluble CTLA4-Fc. or control irrelevant protein TROY-Fc. T cell activation was assessed by measuring IFNγ in the supernatants. Statistical analyses were performed using Student’s t test. Data are from one of two independent experiments.