TABLE 5.

Summary of extraction methods tested.

Method	Key features	Reagent cost (per sample)	Advantages	Disadvantages	References*
Phenol:Chloroform	•  Beadbeating •  CTAB for lysis •  Heat to 65°C	$3.85	•  Does not require special equipment (Kingfisher Flex) •  High DNA yield	•  Contamination in negative controls •  Labor intensive •  Uses organic solvent requiring additional worker safety precautions	Fujimura et al., 2014; Levin et al., 2016; O’Connor et al., 2018; Levan et al., 2019; Rackaityte et al., 2020
Promega Maxwell HT 96 gDNA Blood Isolation (Technical Manual #TM473 per manufacturer)	•  Beadbeating •  CTAB for lysis •  Heat to 95°C •  Proteinase K	$4.12	•  High-throughput •  High DNA yield •  Low bias	•  Requires special equipment (Kingfisher Flex)	Petrof et al., 2013; Levin et al., 2016; Sinha et al., 2017; Chapagain et al., 2019; Clarke et al., 2019
Qiagen MagAttract PowerSoil DNA KF Kit (Earth Microbiome Protocol)	•  Beadbeating •  SDS for lysis •  Heat to 65°C •  RNase A	$6.27	•  High-throughput	•  Requires special equipment (Kingfisher Flex) •  High cost	Easson and Thacker, 2014; McDonald et al., 2016; Halfvarson et al., 2017; Marotz et al., 2017; McDonald et al., 2018
ZymoBIOMICS 96 Magbead DNA Kit (Manufacturer instructions)	•  Beadbeating •  Proprietary lysing agent •  Heat to 55°C •  Proteinase K	$5.41	•  High-throughput	•  Requires special equipment (Kingfisher Flex) •  Lower DNA yield •  Some samples failed library prep	Terranova et al., 2018; Wurm et al., 2018; Zabat et al., 2018; Gibson et al., 2019; Reinhart et al., 2019

*This list is not exhaustive. Example references include five studies from a variety of journals, study types, and sample types.