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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Cancer Cell. 2008 Sep 9;14(3):212–225. doi: 10.1016/j.ccr.2008.07.017

Figure 8. Elevated Ras Activity Incorporated with the EGFR Pathway in IKKα-null Keratinocytes.

Figure 8.

(A) Ras (GTP) activity in keratinocytes, as detected by GST-pull down and Western blotting; elevated Ras and ERK activities were down-regulated by IKKα, inactivation of Ras, and EGFR inhibitor. +/+, Ikkα+/+; −/−, Ikkα−/− keratinocytes; Ras-GTP, active form of Ras; Ras, p-ERK, and β-Actin, detected by Western blotting; AG1478, EGFR inhibitor; N17, domain negative form of Ras; V61, active form of Ras.

(B) Filaggrin expression in Ikkα−/− keratincoytes affected by indicated proteins, as detected by Western blotting. β-Actin, loading control.

(C) Colony formation of Ikkα−/− keratincoytes affected by indicated proteins, stained with 0.5% crystal violet blue.

(D) Filaggrin expression in Ikkα+/+ keratincoytes affected by indicated proteins, as detected by Western blotting.

(E) A work hypothesis of IKKα function in keratinocytes for maintaining skin homeostasis and preventing skin cancer. Arrows, promotion; line, inhibition.