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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: J Vis Exp. 2019 Apr 3;(146):10.3791/59243. doi: 10.3791/59243

Figure 1.

Figure 1.

Assay set up for SWIP. Gravid adult wild-type (N2) worms were lysed with sodium hypochlorite/NaOH treatment to release embryos. The embryos were allowed to hatch and develop into synchronized L1 larvae in M9 buffer for 14 hours on a shaker and then plated on an NGM plate seeded with NA22 bacteria. After 42-48 hours late L4 stage larvae were visually identified under the stereoscope and picked with an eyelash pick into a spot plate with or without amphetamine in control sucrose solution and scored for SWIP either manually or through automated analysis.