Figure 7.

Western blot analysis and immunofluorescence of αSMA expression in HS-derived fibroblasts. HS- derived fibroblasts were pre-treated with hTGF-β1 (5 ng/mL) followed by ALK5 inhibition, subdivided into exon skipping (ALK5ViM [2 µM]) (a) and SB431542 (10 µM) (b). After 24 h, samples were harvested for protein analysis or fixed for immunofluorescence (c). (a, b) The mean relative band intensity of αSMA was calculated by dividing the relative αSMA band intensity by its corresponding relative β-actin (loading control) band intensity. n = 3, error bars represent standard error of the mean. (c) Immunofluorescence for αSMA (red) and merged images show αSMA DAPI nuclear staining (blue). These images are representative for two separate experiments. Scalebar: 100 µm. HS, hypertrophic scar.