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. 2020 Jun 1;34(11-12):819–831. doi: 10.1101/gad.335471.119

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© 2020 Lamothe et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — Cdc5p is recruited by cohesin and brought to condensin at centromeres and rDNA. (A) Cdc5p is recruited by cohesin and brought to condensin at centromeres. ChIP-seq signals from a representative portion of chromosome XVI are presented with bar charts. The region presented includes the centromere (CEN black circle) and the pericentric region (peri-CEN black box). Signal coming from condensin subunit Ycg1p-Myc in wild-type strain is in green, signal from cohesin subunit Mcd1p in wild-type strain is in blue, signal coming from Cdc5p-V5 is in orange, and signal coming from GFP-control is in gray. Cdc5p ChIP-seq signal from wild type is in the first orange lane, from cells depleted of cohesin subunit Mcd1p-AID from G1 to mid-M is in the second orange lane, and from cells depleted of cohesin subunit Mcd1p-AID in mid-M is in the third orange lane. The black arrow indicates enrichment of Cdc5p at the centromere when cohesin is transiently associated with chromosomes. The scale is 0–15 for Ycg1p, 0–21 for Mcd1p, and 0–17 for Cdc5p. (B) Cdc5p is recruited by cohesin and brought to condensin at RDN. ChIP-seq signals from a portion of chromosome XII are presented with bar charts. ChIP-seq signal from strains as in A. The region presented includes one copy of the rDNA repeat (9.1 kb) made of the transcribed regions RND37 and RND5 (black box with white arrows indicating the direction of transcription) and the nontranscribed regions NTS1 and NTS2 (black line). Region of enrichment of cohesin signal is marked by two blue dashed lines (from NTS2 to NTS1). Region of enrichment of condensin signal is marked by two green dashed lines (end of NTS1). Black arrow indicates enrichment of Cdc5p at the condensin-bound region of the RDN when cohesin is transiently associated with chromosomes. The scale is 0–5200 for Ycg1p, 0–2300 for Mcd1p, and 0–1700 for Cdc5p.