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. 2020 Jun 1;34(11-12):785–805. doi: 10.1101/gad.335836.119

Figure 4.

Figure 4.

Expression of ALS mutant FUS or overexpression of wild-type FUS represses protein levels of target RCC transcripts. (A) GFP-FUS and indicated mutant derivatives were transiently expressed in HEK293T cells for 48 h. Western blot (WB) of FUS targeted NADH dehydrogenase subunits. (B) WB quantification of FUS targeted NADH dehydrogenase subunit levels in HEK293T expressing GFP-tagged wild-type FUS and mutant derivatives. Protein levels are normalized to actin. Data are shown in mean with SE from three independent experiments. (*) P < 0.05; (**) P < 0.01; (***)P < 0.001, unpaired t-test. (C) WB of proteins encoded by transcripts that are not enriched in the insoluble mutant FUS IP. (D) WB analysis of relative protein levels in C. Data are displayed in mean with SE from three independent experiments. Protein levels are normalized to GAPDH. Statistics: unpaired t-test. (E) Total RCC mRNA levels were determined by RT-qPCR. Relative mRNA levels are normalized to GAPDH. Data are displayed in mean with SD from three experiments. Statistics: unpaired t-test. (F) WB of NADH dehydrogenase subunit expression levels in HEK293T transfected with siFUS for 72 h. (G) WB quantification of NADH dehydrogenase subunits in F. Protein levels are normalized to actin. Data are shown in mean with SD from three independent experiments. Statistics: unpaired t-test.