Skip to main content
. Author manuscript; available in PMC: 2020 Oct 6.
Published in final edited form as: Nat Chem Biol. 2020 Apr 6;16(5):570–576. doi: 10.1038/s41589-020-0507-z

Fig. 4 |. Simultaneous decoding of three unnatural codons.

Fig. 4 |

a, Schematic illustration of gene cassette containing sfGFP151,190,200(AXC,GXT,AGX), M. mazei tRNAPyl(XCT), M. jannaschii tRNApAzF(GYT), and E. coli tRNASer(AYC). b, Time-course plot of normalized fluorescence during sfGFP expression in the absence or presence of AzK and/or pAzF (aTc added at t = 0; n = 3, biological replicates; mean and individual data points shown). c, Representative deconvoluted mass spectrum from HRMS ESI-TOF analysis of intact sfGFP purified from SSOs in b. Peak labels denote molecular weight as well as quantification of each peak relative to other relevant species (Supplementary Fig. 8). Standard single-letter amino acid code used. Mean ± standard deviation shown (n = 3).