Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May;30(5):757–767. doi: 10.1101/gr.249045.119

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Chang et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 2. — Design and construction of the CRISPR sgRNA library for B. mori cells. (A) Flowchart for constructing the CRISPR sgRNA library for B. mori cells. (B) Design for all sgRNAs in all B. mori gene exons (lower), and the sgRNAs selected for the subsequent experiment (upper); see Supplemental Table S2. (C) Second-generation sequence analysis of the sgRNA distribution for the pB-CRISPR library (orange) and BmEGCKLib (green). (D) Pie chart showing the percent distribution of the sgRNAs. (E) Overlap in sgRNA distribution between the pB-CRISPR library and BmEGCKLib. (F) Correlation analysis between the pB-CRISPR library and BmEGCKLib.