Chemicals, Peptides, and Recombinant Proteins |
hexanes (98.5%) |
Sigma Aldrich |
178918 |
methanol (99.8%) |
Sigma Aldrich |
179337 |
toluene (99.5%) |
Sigma Aldrich |
179418 |
dioctylamine (98%) |
Sigma Aldrich |
(now available as: see below) |
dioctylamine (97%) |
Sigma Aldrich |
D201146 |
hexyl isothiocyanate (95%) |
Sigma Aldrich |
475912 |
1-octadecene (90%) |
Sigma Aldrich |
O806 |
octadecylamine (99%) |
Sigma Aldrich |
74750 |
oleic acid (99%) |
Sigma Aldrich |
O1008 |
selenium pellets (>99.99%) |
Sigma Aldrich |
209643 |
zinc acetate dihydrate (>99%) |
Sigma Aldrich |
96459 |
cadmium oxide (99.99%) |
Strem Chemicals |
93-4817 |
tri-n-butylphosphine (99%) |
Strem Chemicals |
(now available as: see below) |
tri-n-butylphosphine (min. 93%) |
Strem Chemicals |
15-5801 |
tri-n-octylphosphine (97%) |
Strem Chemicals |
15-6655 |
Alexa-594 |
Sigma-Aldrich |
N/A |
4-Aminopyridine |
Sigma-Aldrich |
Cat # 275875; CAS:504-24-5 |
3M KCl |
Sigma-Aldrich |
N/A |
Experimental Models: Organisms/Strains |
C57BL/6 wildtype |
Jackson Laboratory |
RRID:IMSR_JAX:000664 |
C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J |
Jackson Laboratory |
RRID:IMSR_JAX:024276 |
Thy-1 |
Jackson Laboratory |
RRID:IMSR_JAX:000406 |
PV-Cre |
Jackson Laboratory |
RRID:IMSR_JAX:017320 |
LSL-TOM |
Jackson Laboratory |
RRID:IMSR_JAX:007908 |
Recombinant DNA |
AAV1-Syn-GCaMP6s-WPRE-SV40 |
U Penn Vector Core |
Cat # AV-1-PV2824 |
Software and Algorithms |
ImageJ |
https://imagej.nih.gov/ij/download.html |
N/A |
Moco |
(Dubbs et al., 2016) |
N/A |
MATLAB(R2014b) |
MathWorks |
https://www.mathworks.com |
COMSOL |
COMSOL (USA) |
N/A |
Adobe Illustrator CS6 |
Adobe |
N/A |
Custom lab view program for in vitro data acquisition |
PackIO (Packer, 2011) |
N/A |
Priarie View Software for in vivo data acquisition |
Bruker Corporation |
N/A |
Other |
Custom built Ultima In Vivo two-photon microscope |
Bruker Corporation |
Model: U-IV-02 |
Tunable Ti:Sapphire laser |
Coherent |
Model: Chameleon Ultra II |
Pockels cell |
Conoptics |
Model # 350-160BK |
Pockels cell driver |
Conoptics |
Model # 302 RM |
MultiClamp 700B amplifier+commander |
Molecular Devices |
Model # 2500-0157 |
Custom built Olympus In Vitro microscope |
Olympus |
N/A |
Atomic Force Microscope |
Bruker Corporation |
Bruker Catalyst |
P-2000 laser puller |
Sutter Instruments, USA |
P-2000 |
Quartz capillaries 1mm * 0.5mm |
Sutter Instruments, USA |
N/A |