Figure 1.

CCR2⁺ Mo-DCs and Neutrophils Produce CXCL9 and CXCL10 during A. fumigatus Challenge

(A–C) Lung CXCL9 and CXCL10 levels in (A) C57BL6 mice (n = 5) at 0–72 h pi or (B and C) in DT-treated CCR2-DTR^+/− and non-Tg (CCR2-DTR^−/−) littermates (n = 5) at 48 h pi with 3 × 10⁷ CEA10 conidia.

(D) Representative plots of RFP (CXCL9) and BFP (CXCL10) expression in indicated lung leukocytes isolated from Rex3 Tg → C57BL6.SJL BM chimeric mice at baseline (naive, top row) and 48 h pi with 3 × 10⁷ CEA10 conidia (infected, bottom row). The blue and purple gates indicate the frequency of BFP⁺ (CXCL9⁺) and BFP⁺RFP⁺ (CXCL9⁺ CXCL10⁺) cells, respectively.

(E and F) (E) The graphs indicate the frequency of CXCL9⁻ CXCL10⁺ and CXCL9⁺ CXCL10⁺ neutrophils, monocytes, Mo-DCs, and pDCs and (F) the cellular identity of all CXCL9⁻ CXCL10⁺ (top) and CXCL9⁺ CXCL10⁺ lung leukocytes (bottom) at 48 h pi.

(G and H) Lung (G) CXCL9 and (H) CXCL10 levels in DT-treated ROSA26-iDTR^Mrp8-Cre → C57BL6.SJL (neutrophil depleter) or non-Cre ROSA26-iDTR littermates → C57BL 6.SJL (control) BM chimeric mice (n = 10) at 48 h pi with 3–4 × 10⁷ heat-killed swollen CEA10 conidia. Data from two independent experiments were pooled.

(A–C, G, and H) Dots represent individual mice and data are presented as mean ± SEM. Statistical analysis: (A) Kruskal-Wallis test, time points compared with t = 0 h, (B, C, G, and H) Mann-Whitney test. See also Figure S1.