Antibodies |
|
APC Mouse anti-Mouse CD45.1 (clone A20) |
BD Bioscience |
Cat# 558701; RRID: AB_1645214
|
PerCP-Cy™5.5 Mouse anti-Mouse CD45.2 (clone 104) |
BD Bioscience |
Cat# 552950; RRID: AB_394528
|
APC-Cy™7 Rat anti-Mouse CD45 (clone 30-F11) |
BD Bioscience |
Cat# 557659; RRID: AB_396774
|
PerCP-Cy™5.5 Rat anti-CD11b (clone M1/70) |
BD Bioscience |
Cat# 550993; RRID: AB_394002
|
PE-Cy™7 Hamster anti-Mouse CD11c (clone HL3) |
BD Bioscience |
Cat# 558079; RRID: AB_647251
|
BV 605™ anti-mouse CD11c Antibody (clone N418) |
BioLegend |
Cat# 117334; RRID: AB_117334
|
PE-Cy™7 Rat Anti-Mouse Ly-6C (clone AL-21) |
BD Bioscience |
Cat# 560593; RRID: AB_1727557
|
FITC Rat anti-Mouse Ly-6G (clone 1A8) |
BD Bioscience |
Cat# 551460; RRID: AB_394207
|
FITC Rat anti-Mouse CD45R/B220 (clone RA3-6B2) |
BD Bioscience |
Cat# 553087; RRID: AB_394617
|
PE anti-mouse CD183 Antibody (clone CXCR3-173) |
BioLegend |
Cat# 126505; RRID: AB_1027656
|
BV421 Rat Anti-Mouse Siglec-F (clone E50-2440) |
BD Bioscience |
Cat# 562681; RRID: AB_2722581
|
BV605 Rat Anti-Mouse Siglec-H (clone 440c) |
BD Bioscience |
Cat# 747673; RRID: AB_2744234
|
Purified Rat Anti-Mouse CD16/CD32 (clone 2.4G2) |
BD Bioscience |
Cat# 553142; RRID: AB_394657
|
eFluor 450 NK1.1 Monoclonal Antibody (clone PK136) |
eBioscience |
Cat# 48-5941-80; RRID: AB_2043878
|
eFluor 450 CD19 Monoclonal Antibody (clone eBio1D3) |
eBioscience |
Cat# 48-0193-82; RRID: AB_2734905
|
Alexa Fluor 700, I-A/I-E Monoclonal Antibody (clone M5/114.15.2) |
eBioscience |
Cat# 56-53-21; RRID: AB_494009
|
FITC Mouse IgG1 kappa Isotype Control |
eBioscience |
Cat# 11-4714-42; RRID: AB_10596964
|
PE Rat IgG2a kappa Isotype Control |
eBioscience |
Cat# 12-4321-42; RRID: AB_1518773
|
PerCP-Cyanine5.5 IgG Isotype Control |
eBioscience |
Cat# 45-4888-80; RRID: AB_906260
|
APC Rat IgG2b kappa Isotype Control |
eBioscience |
Cat# 17-4031-82; RRID: AB_470176
|
APC-eFluor 780 Rat IgG2b kappa Isotype Control |
eBioscience |
Cat# 47-4031-80; RRID: AB_1272021
|
Alexa Fluor 700 Rat IgG2b kappa Isotype Control |
eBioscience |
Cat# 56-4031-80; RRID: AB_837123
|
PE-Cyanine7 Rat IgG2a kappa Isotype Control |
eBioscience |
Cat# 25-4321-82; RRID: AB_470200
|
eFluor 450 Rat IgG1 kappa Isotype Control |
eBioscience |
Cat# 48-4301-82; RRID: AB_1271984
|
Bacterial and Virus Strains |
|
AF293 |
Fungal Genetics Stock Center |
Cat# A1100 |
AF293 ds-Red |
(Jhingran et al., 2012) |
N/A |
CEA10 (also known as CBS144.89) |
(Girardin et al., 1993) |
Received from Robert A. Cramer |
|
Chemicals, Peptides, and Recombinant Proteins |
|
Alexa Fluor 633 succinimidyl ester |
Invitrogen |
Cat# S12375 |
Collagenase IV |
Thermo Fisher Scientific |
Cat# LS004189
|
RPMI-1640 |
RPMI-1640 |
21870092 |
Enrofloxacin |
Bayer |
Baytril 100 |
Voriconazole |
Pfizer |
N/A |
Tween-20 |
Sigma |
P9416 |
Protease Inhibitor Cocktail |
Roche |
Cat# 11697498001 |
DNase I, Grade II |
Sigma |
Cat# 10104159001 |
BCG albumin assay kit |
Sigma |
MAK124 |
Isoflurane |
Henry Schein Animal Health |
Cat# 29405 |
Diphtheria toxin (DT) |
List Biological Laboratories |
Cat# 150 |
Paraformaldehyde, 32% Solution |
Thermo Fisher Scientific |
Cat# 50-980-495 |
CM-H2DCFDA |
Thermo Fisher Scientific |
Cat# C6827 |
10x HBSS |
Thermo Fisher Scientific |
14065056 |
TRIzol-LS |
Invitrogen |
10296028 |
Mouse CXCL9/MIG DuoSet ELISA |
R&D Systems |
DY492 |
Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA |
R&D Systems |
DY466 |
CytoTox96 non-radioactive cytotoxicity assay kit |
Promega |
G1780 |
High Capacity RNA-to-cDNA Kit |
Applied Biosystems |
4387406 |
Ribosomal RNA Control Reagents |
Applied Biosystems |
4308329 |
Cxcl9 ASSAY ID: Mm01345159_m1 |
Thermo Fisher |
4448892 |
Cxcl10 ASSAY ID: Mm00445235_m1 |
Thermo Fisher |
4453320 |
|
Experimental Models: Organisms/Strains |
|
Cxcr3-/-
|
The Jackson Laboratory |
JAX: 005796 |
BDCA2-DTR |
The Jackson Laboratory |
JAX: 014176 |
Clec7a-/-
|
(Saijo et al., 2007) |
N/A |
Card9-/-
|
(Hsu et al., 2007) |
JAX: 028652 |
Ccr2-/-
|
The Jackson Laboratory |
JAX: 004999 |
Ifnar1-/-
|
The Jackson Laboratory |
JAX: 32045 |
Ifnlr-/-
|
(Lin et al., 2016) |
N/A |
C57BL6 |
The Jackson Laboratory |
JAX: 000664 |
C57BL6.SJL |
Charles River Laboratories |
Stock No. 564 |
Rag2-/-Il2rg-/-
|
Taconic |
Stock No. 4111 |
MRP8-Cre-IRES/GFP |
The Jackson Laboratory |
JAX: 021614 |
ROSA26 iDTR |
The Jackson Laboratory |
JAX: 007900 |
CCR2-DTR |
(Hohl et al., 2009) |
N/A |
REX3 Tg |
(Groom et al., 2012) |
N/A |
|
Software and Algorithms |
|
Prism 8 |
Prism 8 |
N/A |
FlowJo 9.9.6 |
FlowJo 9.9.6 |
N/A |