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. 2020 Jun 1;28(1):104–116.e4. doi: 10.1016/j.chom.2020.05.002
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

APC Mouse anti-Mouse CD45.1 (clone A20) BD Bioscience Cat# 558701; RRID: AB_1645214
PerCP-Cy™5.5 Mouse anti-Mouse CD45.2 (clone 104) BD Bioscience Cat# 552950; RRID: AB_394528
APC-Cy™7 Rat anti-Mouse CD45 (clone 30-F11) BD Bioscience Cat# 557659; RRID: AB_396774
PerCP-Cy™5.5 Rat anti-CD11b (clone M1/70) BD Bioscience Cat# 550993; RRID: AB_394002
PE-Cy™7 Hamster anti-Mouse CD11c (clone HL3) BD Bioscience Cat# 558079; RRID: AB_647251
BV 605™ anti-mouse CD11c Antibody (clone N418) BioLegend Cat# 117334; RRID: AB_117334
PE-Cy™7 Rat Anti-Mouse Ly-6C (clone AL-21) BD Bioscience Cat# 560593; RRID: AB_1727557
FITC Rat anti-Mouse Ly-6G (clone 1A8) BD Bioscience Cat# 551460; RRID: AB_394207
FITC Rat anti-Mouse CD45R/B220 (clone RA3-6B2) BD Bioscience Cat# 553087; RRID: AB_394617
PE anti-mouse CD183 Antibody (clone CXCR3-173) BioLegend Cat# 126505; RRID: AB_1027656
BV421 Rat Anti-Mouse Siglec-F (clone E50-2440) BD Bioscience Cat# 562681; RRID: AB_2722581
BV605 Rat Anti-Mouse Siglec-H (clone 440c) BD Bioscience Cat# 747673; RRID: AB_2744234
Purified Rat Anti-Mouse CD16/CD32 (clone 2.4G2) BD Bioscience Cat# 553142; RRID: AB_394657
eFluor 450 NK1.1 Monoclonal Antibody (clone PK136) eBioscience Cat# 48-5941-80; RRID: AB_2043878
eFluor 450 CD19 Monoclonal Antibody (clone eBio1D3) eBioscience Cat# 48-0193-82; RRID: AB_2734905
Alexa Fluor 700, I-A/I-E Monoclonal Antibody (clone M5/114.15.2) eBioscience Cat# 56-53-21; RRID: AB_494009
FITC Mouse IgG1 kappa Isotype Control eBioscience Cat# 11-4714-42; RRID: AB_10596964
PE Rat IgG2a kappa Isotype Control eBioscience Cat# 12-4321-42; RRID: AB_1518773
PerCP-Cyanine5.5 IgG Isotype Control eBioscience Cat# 45-4888-80; RRID: AB_906260
APC Rat IgG2b kappa Isotype Control eBioscience Cat# 17-4031-82; RRID: AB_470176
APC-eFluor 780 Rat IgG2b kappa Isotype Control eBioscience Cat# 47-4031-80; RRID: AB_1272021
Alexa Fluor 700 Rat IgG2b kappa Isotype Control eBioscience Cat# 56-4031-80; RRID: AB_837123
PE-Cyanine7 Rat IgG2a kappa Isotype Control eBioscience Cat# 25-4321-82; RRID: AB_470200
eFluor 450 Rat IgG1 kappa Isotype Control eBioscience Cat# 48-4301-82; RRID: AB_1271984
Bacterial and Virus Strains

AF293 Fungal Genetics Stock Center Cat# A1100
AF293 ds-Red (Jhingran et al., 2012) N/A
CEA10 (also known as CBS144.89) (Girardin et al., 1993) Received from Robert A. Cramer

Chemicals, Peptides, and Recombinant Proteins

Alexa Fluor 633 succinimidyl ester Invitrogen Cat# S12375
Collagenase IV Thermo Fisher Scientific Cat# LS004189
RPMI-1640 RPMI-1640 21870092
Enrofloxacin Bayer Baytril 100
Voriconazole Pfizer N/A
Tween-20 Sigma P9416
Protease Inhibitor Cocktail Roche Cat# 11697498001
DNase I, Grade II Sigma Cat# 10104159001
BCG albumin assay kit Sigma MAK124
Isoflurane Henry Schein Animal Health Cat# 29405
Diphtheria toxin (DT) List Biological Laboratories Cat# 150
Paraformaldehyde, 32% Solution Thermo Fisher Scientific Cat# 50-980-495
CM-H2DCFDA Thermo Fisher Scientific Cat# C6827
10x HBSS Thermo Fisher Scientific 14065056
TRIzol-LS Invitrogen 10296028
Mouse CXCL9/MIG DuoSet ELISA R&D Systems DY492
Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA R&D Systems DY466
CytoTox96 non-radioactive cytotoxicity assay kit Promega G1780
High Capacity RNA-to-cDNA Kit Applied Biosystems 4387406
Ribosomal RNA Control Reagents Applied Biosystems 4308329
Cxcl9 ASSAY ID: Mm01345159_m1 Thermo Fisher 4448892
Cxcl10 ASSAY ID: Mm00445235_m1 Thermo Fisher 4453320

Experimental Models: Organisms/Strains

Cxcr3-/- The Jackson Laboratory JAX: 005796
BDCA2-DTR The Jackson Laboratory JAX: 014176
Clec7a-/- (Saijo et al., 2007) N/A
Card9-/- (Hsu et al., 2007) JAX: 028652
Ccr2-/- The Jackson Laboratory JAX: 004999
Ifnar1-/- The Jackson Laboratory JAX: 32045
Ifnlr-/- (Lin et al., 2016) N/A
C57BL6 The Jackson Laboratory JAX: 000664
C57BL6.SJL Charles River Laboratories Stock No. 564
Rag2-/-Il2rg-/- Taconic Stock No. 4111
MRP8-Cre-IRES/GFP The Jackson Laboratory JAX: 021614
ROSA26 iDTR The Jackson Laboratory JAX: 007900
CCR2-DTR (Hohl et al., 2009) N/A
REX3 Tg (Groom et al., 2012) N/A

Software and Algorithms

Prism 8 Prism 8 N/A
FlowJo 9.9.6 FlowJo 9.9.6 N/A