Table 2. Characteristics (the 5’ end labeling fluorophores; F: the forward primer sequences; R: the reverse primer sequences; Ta: annealing temperature; sizes of the amplified fragments in base pairs-bp) of the microsatellites (SSRs) developed for Fusarium oxysporum f. sp. phaseoli isolates.

SSR/ fluorophores	Primer sequences (5’-3’)	Motifs	Number of Alleles	TA (°C)	Sizes of the amplified Fragments (bp)	PIC	GenBank Accession number
	FOP 01-B01 NED	F – CCGCCGATTTCCTTACCT	(AT)9	03	58	237-254	0.13	MK622886
R - GCACCCTTCAAACCTCCA
FOP 07-B01 PET	F - TGAGCGAATGGGAAGAGG	(GGAG)5	06	58	151-225	0.54	MK622887
	R - TGCCAAGGGAGTATCATTTC
FOP 10-B01 PET	F – ATGGGATAGGCGGTTTGG	(TC)3/(AG)3	05	58	236-255	0.77	MK602310
	R - ACTTGGGGTTGAGCGTTG
FOP 13-B01 6FAM	F - TTCTTGCTGATCGCCTCA	(GGCAT)5	02	59	152-167	0.37	MK602312
	R - CCTCCCCTGCAATAAGAGC
FOP 15-B01 NED	F - CCGTCTTCATCTTGGCGTCTA	(AGAT)4	03	54	209-225	0.09	MK622888
	R - TATCTAAGGGGTGGGACGGAG
FOP 16-B01 PET	F - CCGCGCTCTCGAGCAGGG	(ACT)3	02	54	202-205	0.35	MK602313
	R - TGCCGAAGTAAAAGCACGG
FOP 20-B01 6-FAM	F - CCGTTTGGCGCAAGTT	(GA)6	10	54	148-188	0.70	MK622889
	R - CGAGCGGCTTGTCTTCA
FOP 28-B01 PET	F - GTTGCGCTCCCCAATATG	(TC)3/(AT)3	02	56	204-208	0.09	MK622890
	R - CCTCCATTGCCATCCATTT
FOP 35-B01 NED	F - GGCTGGTGGTTTCAAGAGAG	(CAC)6	06	58	210-236	0.40	MK622891
	R – CAAGGCTTCTTCACGGGTAA
FOP 37-B01 NED	F - GCAACACAGGAGGTGGTA	(TGCT)3	04	54	180-192	0.29	MK622892
	R - CAAGGGTATTGGTGCTTG
FOP 08-B02 6-FAM	F - ACAGTGGCTCGTGACTCCA	(CT)3(TA)3	06	59	222-240	0.38	MK602309
	R - TGTTGGCACAGAGGCAGA
FOP 09-B02 NED	F - TGCAGCGATGAGATTGGA	(CCA)3	03	54	165-169	0.35	MK622893
	R - GCGTCATCTCGTGAATCG
FOP 11-B02 VIC	F - AACAGCCGAAGCCGATG	(AG)7	07	59	259-305	0.62	MK602311
	R - TTCACCTTCCACTTGCACA
FOP 18-B02 PET	F - AATGCGGCCACTGTGATT	(TCA)3/(ATC)2	04	59	151-164	0.33	MK602314
	R - GCAGAAGTTGCGGTTGTG
FOP 23-B02 NED	F - CAGGGGCACAGTTCGGTA	(TC)3/(AT)3	04	59	190-196	0.35	MK622894
	R - ATCGGCTGGGACATGAAG