| Stage | Temperature (°C) | Time (min:sec) | Cycles |
|---|---|---|---|
| 1 | 50 | 2:00 | 1 |
| 2 | 95 | 10:00 | 1 |
| 3 | 95 | 0:15 | 40 |
| 4 | 60 | 1:00 | |
| 5 | 95 | 0:15 | 1 |
| 6 | 60 | 1:00 | 1 |
| 7 | 95 | 0:15 | 1 |
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