| Tube # | Final Concentration (ng/μl) | Volume (μl) BSA 2000 μg/ml | Volume 2x SDS Sample Buffer (μl) | Volume H2O (μl) |
|---|---|---|---|---|
| 1 | 1000 | 200 | 200 | 0 |
| 2 | 750 | 150 | 200 | 50 |
| 3 | 500 | 100 | 200 | 100 |
| 4 | 250 | 50 | 200 | 150 |
| 5 | 125 | 25 | 200 | 175 |
| 6 | 25 | 5 | 200 | 195 |
| 7 | 0 | 0 | 200 | 200 |
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