Protocol	Total time	Details
Support Protocol 1: Production of HPV Minicircles	4 days	Day 1: 2 hours
		Day 2: 30 minutes
		Day 3: 8 hours
		Day 4: 2 hours
Alternate Support Protocol 1: Production of Recircularized HPV Genomes	3 days	Day 1: 1.5 hours
		Day 2: 15 minutes
		Day 3: 2 hours
Basic Protocol 1: Transfection, Harvest, and Isolation of HPV Quasiviruses	6 days	Day 1: 30 minutes
		Day 2: 1 hour
		Day 3: 30 minutes
		Day 4: 1.5 hours
		Day 5: 6 hours
		Day 6: 1 hour
Alternate Protocol 1: Packaging HPV DNA Replicated in 293TT Cells	1 day	Day 1: 1 hour
Alternate Protocol 2: Production of Higher Purity Quasivirus Using “Ripcord” Method	2 days	Day 1: 1.5 hours
		Day 2: 1.5 hours
Support Protocol 2: Screening of Fractions for Viral Proteins	2 Days	Day 1: 3 Hours
		Day 2: 30 minutes
Support Protocol 3: Screening of Fractions for Viral DNA	1 day	Day 1: 3 hours
Support Protocol 4: Measuring Viral Titer	1 day	Day 1: 4 hours
Support Protocol 5: Quantitation of HPV Quasivirions	2 Days	Day 1: 3 hours
		Day 2: 30 minutes
Basic Protocol 2: HPV Quasivirus Infections of Primary Human Foreskin Keratinocytes	3 days, not including time points post-infection	Day 1: 1 hour
		Day 2: 30 minutes
		Day 3: 2 hours
Basic Protocol 3: HPV Quasivirus Transcription Assay	1 day	Day 1: 5 hours
Basic Protocol 4: HPV Quasivirus Replication Assay	1 day	Day: 6 hours