Inferring leading interactions in the p53/Mdm2/Mdmx circuit through live-cell imaging and modeling

. Author manuscript; available in PMC: 2020 Dec 18.

Published in final edited form as: Cell Syst. 2019 Dec 4;9(6):548–558.e5. doi: 10.1016/j.cels.2019.10.010

Key Resource Table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mdm2 (SMP₁₄)	Santa Cruz	Cat. No. sc-965
P53 (FL-393)	Santa Cruz	Cat. No. sc-6243
Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488	ThermoFisher Scientific	Cat. No. A32723
Donkey anti-Rabbit IgG (H+L) Highly Cross-Ad-sorbed Secondary Antibody, Alexa Fluor Plus 647	ThermoFisher Scientific	Cat. No. A32795
DharmaFect I	Dharmacon	Cat. No. T-2001
Chemicals
DAPI	Sigma	D9542
DABCO 33-LV	Sigma	Cat. No. 290734
Experimental Models: Cell Lines
MCF7+p53shRNA+p53-mCerulean	(Gaglia et al., 2013)	N/A
Oligonucleotides
*MDMX* siRNA: AGCCCTCTCTATGATATGCTA	Qiagen	Cat. No. 1027417
*MDMX* siRNA: GACCACGAGACGGGAACATTA	Qiagen	Cat. No. 1027417
AllStars Negative Control siRNA	Qiagen	Cat. No. 1027280
Software and Algorithms
MATLAB 2019b	The MathWorks, Inc.	https://www.math-works.com
P53 Cinema Single Cell Tracking Software	(Reyes et al., 2018)	https://github.com/balvahal/p53Cine-maManual
Custom Matlab script- model	This work	https://github.com/Mathiasheltberg/In-teractionsP53Net-work