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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Neuropharmacology. 2019 Apr 6;158:107596. doi: 10.1016/j.neuropharm.2019.04.002

Table 1.

MRM transitions, cone voltages, collision energies and retention times.

Analyte MRM
Transitions		 MS/MS parameters Retention time (RT) a
Cone
voltage
(V)		 Collision
energy
(eV)		 RT CV
(%)
Carfentanil 395.1 > 335.2 12 16 5.4 0.7
395.1 > 113.1 12 28
Norcarfentanil 291.2 > 142.3 8 14 2.7 0.4
291.2 > 113.3 8 26
Carfentanil-d5 400.2 > 330.2 12 16 5.4 0.2
400.2 > 133.1 12 28
Norfentanyl-d5 238.5 > 84.1 18 16 2.5 0.7
238.5 > 155.1 18 16
a

RT was calculated based on 8 assays of calibrators and QC samples.