Figure 2. Anatomical Organization of CeS Neurons.

(A) Experimental strategy for visualizing pre-cervical (CeS^cer) and pre-lumbar (CeS^lum) CeS neurons.

(B) Rostro-caudal (top-bottom) coronal sections showing retrolabeled GFP⁺ CeS^cer neurons (green) and mCherry⁺ CeS^lum neurons (red) in the DCN (outlined). Boxed area in row 2 shows anatomical segregation of CeS^cer and CeS^lum neurons in the IntA. Stitched, Z-projected images are shown.

(C) Quantification of total number of neurons (gray, n = 3 mice, dentate excluded) and the total number of CeS^cer (green, n = 7 mice) or CeS^lum (red, n = 4 mice) neurons in the respective DCN (~20 sections per mouse). Individual values and mean ± SEM are shown.

(D) Summary schematic depicting the main anatomical subdivisions of CeS neurons.

Scale bar, 200 μm. See also Figures S1 and S2.