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[Preprint]. 2020 May 21:2020.05.21.108381. [Version 1] doi: 10.1101/2020.05.21.108381

Fig. 8. Rapid Detection of SARS-CoV- 2 in Viral Transport Media in an additively manufactured cartridge and handheld point of care instrument.

Fig. 8.

(a) Baseline-subtracted fluorescence images of real-time RT-LAMP on the additively manufactured amplification chip at different time points showing the amplification of 5000 copies/μL of SARS-CoV- 2 inactive virus in VTM. (b) Baseline-subtracted fluorescence images of real-time RT-LAMP on the additively manufactured amplification chip at different time points showing the amplification of VTM only negative control. (c) Baseline subtracted mean fluorescent intensity over time for on-chip amplification detection. (d) Amplification chip endpoint fluorescence images at time = 40 min. (for 3 chip replicates) of 5000 copies/μL of SARS-CoV- 2 inactive virus in VTM (top) and, amplification chip endpoint fluorescence images at time = 40 min. of VTM only negative control (bottom). (e) Mean fluorescent intensity at time = 40 min. (for 3 chip replicates) for 5000 copies/μL of SARS-CoV- 2 inactive virus in VTM and VTM only negative control. The bar graphs show mean and standard deviation of the fluorescence intensity of the 6 pools in a single chip replicate.