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[Preprint]. 2021 Apr 29:2020.04.22.056283. Originally published 2020 Apr 23. [Version 2] doi: 10.1101/2020.04.22.056283

PMC7263496.1; 2020 Apr 23
PMC7263496.2; 2021 Apr 29

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Figure 2. — (A) Assay layout of the EF-RT-qPCR test. One sample is assayed in four wells on a 384 well-plate. Each sample is tested for two probes, in duplicate. RNase P is assayed in each well. (B) Combinations of how positive, inconclusive (abbreviated Inc.), negative, and failed samples are determined. (C) Mean Ct values for 67 specimens processed by EF-RT-qPCR and extraction-based RT-qPCR.