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(1) Anterior nares swabs are collected and (2) transported dry to the lab. Upon receipt, (3) each swab is then hydrated with low-TE buffer, aliquoted into a 96 well plate, and (4) proteinase K is added to every well. (5) The eluted specimens are digested and heat-inactivated in a laboratory oven before (6) they are loaded as the template in a RT-qPCR reaction. The cost listed includes reagents and consumables.
