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(A) A micro BCA assay was used to quantify the % RBD adsorbed on Alhydrogel® at different RBD to Alhydrogel® (Al) ratios; (B) SDS-PAGE analysis: the supernatant and pellet fractions for a formulation of 0.2 mg/mL RBD219-N1 with 1.6 mg/mL Alhydrogel (1:8 ratio) were analyzed. 10 μL of the desorbed RBD from the pellet fraction (P) and 10 μL of supernatant (S) were loaded on the gel and stained with Coomassie Blue. M: protein marker.
