Figure 3. Comparison of Alhydrogel (Al) and AddaVax (MF-59-like adjuvant).

(A)qPCR for the expression of the SARS-CoV np gene in the lungs of mice and (B) neutralizing antibody-100 (NT100) titers of mice differentially vaccinated with RBD219-N1 (log₂). The mice (N=4) were vaccinated with RBD219-N1 formulated in Alhydrogel®, AddaVax (MF59-like adjuvant), and no adjuvant. On day 52, sera were collected to evaluate IgG and neutralizing antibody titers. On day 63, mice were i.n. challenged with SARS-CoV. Each bar represented an individual mice, two mice per group were sacrificed on day 3 and day 6 posted infection (dpi) to evaluate viral load.