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. 2020 Jun 1;16(6):e1008717. doi: 10.1371/journal.pgen.1008717

Fig 6. Elimination of integrin in FCs disrupts cytoskeletal organisation in neighbouring control cells.

Fig 6

(A, B) Basal surface view of S10A (A, A’) and S10B (B, B’) mosaic follicular epithelia containing mys FC clones (GFP-negative), stained with anti-GFP (green) and Rhodamine Phalloidin to detect F-actin (red). (C) Lateral view of a S10 mosaic egg chamber stained with anti-GFP (green), Rhodamine Phalloidin (red) and anti-Dlg (basolateral polarity marker Discs large, blue). (D) 3D reconstruction of mys FCs and surrounding control cells. Arrows in C and D point to the basal surface of a mutant FC. (E, E’) Basal surface view of a S10B mosaic FE containing mys FC clones (GFP-negative). Yellow arrows and asterisks mark control FCs contacting control and mys FCs, respectively. (F-G’) Confocal images of live S10B mosaic egg chambers containing mys (F) or GFP (G) clones and expressing the cell membrane marker Resille-GFP. Images were taken with a 40 minutes interval. Scale bars, 5μm.