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. 2020 Jun 1;15(6):e0233860. doi: 10.1371/journal.pone.0233860

Fig 4. Apoptotic cells and functionality of day 135 recovered shipped retinal organoids.

Fig 4

(A) Brightfield images of apoptotic and necrotic cells were detected in control (left image) and shipped organoids (right image), stained with dark brown colour, indicated by red arrows. No significant differences were observed between control and shipped organoids (p = 0.9355). (B) Spike raster plots (top) and firing rate histogram (bottom) from putative OFF retinal ganglion cells (RGCs) of control and shipped d135 organoids revealed that their spiking activity decreased after white light pulses (WLP). Each row in the raster plot (y-axis) represents a different RGC and each vertical bar represents a spike from the corresponding RGC. The red line illustrates the stimulus onset whereas the left half before indicates the spontaneous activity before WLP exposure and the right half when exposed to WLP. Box plot indicates the decreased firing rate (in %) of putative OFF RGCs (top) and ON RGC (bottom) in control and RT condition. In both cases, no significant differences were observed (Mann Whitney test; p = 0.77 for OFF RGCs and p = 0.18 for ON RGCs). The box plot shows the median (red line).