Fig 6. Apoptotic cells, functionality and ultrastructural characteristics of day 160 recovered shipped retinal organoids.

(A) Brightfield images illustrate with red arrows the apoptotic and necrotic cells in control (left image) and shipped organoids (right image), stained with dark brown colour. No significant differences were observed between control and shipped day 165 organoids (p = 0.1888). (B) Spike raster plots (top) and firing rate histogram (bottom) from putative OFF RGCs of control and shipment group (day 160) showed a decreased spiking activity after white light pulses (WLP). Box plot revealed the decreased firing rate (in %) of putative OFF RGCs (top) and ON RGC (bottom) in control and RT condition. There are no significant differences between both conditions (Mann Whitney test; p = 0.56 for OFF RGCs and p = 0.86 for ON RGCs). The box plot shows the median (red line). (C) Ultrastructural analysis of day 160 retinal organoids revealed the presence of connecting cilium (CC), basal body (bb), photoreceptor outer segments (OS), photoreceptors possessing inner segments (IS), outer limiting membrane (OLM) and mitochondria (mt) in both shipped and control organoids.