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. 2020 Jun 1;15(6):e0233961. doi: 10.1371/journal.pone.0233961

Fig 1. Display of IL-23 and p19 alanine variants on yeast.

Fig 1

A) P19 subunit was anchored on yeast surface by fusion to vial V5-tag. P40 was expressed as soluble protein. Antibodies against p19, p40, and V5-tag were used to isolate yeast cells with correct assembly of IL-23 heteromer. B) Normalized binding of IL-23R to wild-type IL-23 and alanine variants of p19 displayed on yeast (n = 1). Binding of wild-type IL-23 is shown in black bar and binding of W137A, L141A, and K145A are indicated by red arrows.