Table 1. Peptide sequences and their characterizations by SPR (n = 1).
Sequence | ka (1/Ms) | kd (1/s) | KD (M) | Competition SPR | ||
---|---|---|---|---|---|---|
%Inhibition | Concentration tested (μM) | |||||
IL-23R | 1.70E+05 | 1.51E-03 | 8.92E-09 | 98.0 | 0.1 | |
23–652 | DTLTKSFYWQMYGST | 3.66E+04 | 2.63E-03 | 7.18E-08 | 0.4 | 3 |
23–644 | HVPDWLDVLNS | 4.93E+04 | 1.29E-01 | 2.62E-06 | Not done | - |
23–437 | FTKEIDSWWLSLMSS | 1.78E+05 | 1.86E+00 | 1.05E-05 | 57.6% | 30 |
23–441 | QKITQVYIIWTYPLLNDV | 1.90E+05 | 1.74E-01 | 9.11E-07 | 100.0% | 30 |
23–446 | GLRERVRNYGHEFQNWYW | 3.88E+06 | 8.63E-02 | 2.23E-08 | 79.1% | 3 |
23–447 | LELYYLRWKVDN | 4.83E+04 | 1.33E+00 | 2.75E-05 | 78.7% | 30 |
23–443 | LLQTLQMTSDARTMLWSI | - | - | *NB | - | - |
* NB: not binding. Chemically synthesized peptide did not retain binding as free peptide.