Figure 4.

Generation of Laidx^-/Y and Laidx^Dup/Y transgenic mice. (A) Schematic representation of the mouse X chromosome. Ampliconic regions are shown in blue, centromere in gray, and pseudoautosomal region (PAR) in green. The region of the X chromosome carrying the Laidx-containing amplicons is expanded to show a representation of the repeat structure (blue arrows). Red arrows denote loxP sites. Mice carrying loxP sites flanking the ampliconic region were mated to Ella-Cre mice to generate Laidx^-/Y mice. (B) RT-PCR on RT (+) and no RT controls (-) performed on RNA isolated from adult testis from WT and Laidx^-/Y mice. Trim42 is a testis-specific gene used as a positive control. Primer pairs for each assay are indicated (see Supplemental Tables 1 and 2). (C) Sanger sequencing chromatograms from Laidx RT-PCR product in WT (top) and Laidx^-/Y (bottom) mice. The WT product contains multiple sequence variants that are specific to both the X and Y chromosome. The Laidx^-/Y product contains only variant sequences specific to the Y chromosome. (D) mRNA-seq was performed on testes from WT, Laidx^-/Y, and Laidx^Dup/Y mice.