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Sequence analysis of CRISPR/Cas9 edited proxy genes. CRISPR/Cas9 induced mutations detected via PCR and Sanger sequencing in jar1 (A), gl1 (B) and ein2 (C) mutants across independent T2 progenies. The target sequence is depicted in red. The PAM site is depicted in blue. Indels are depicted in green. Wild type reference DNA sequence (WT) and mutant alleles (M1…5) detected for each proxy gene are shown as sequence alignments.
